General Western Blot Reagents
Azure Biosystems AzureSpectra Labeling Kit, for Western Blot
Kit for covalently labeling antibodies for Western blot with a fluorescent dye. Contains labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns. Each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody
Azure Biosystems HRP Stripping Buffer
Stripping buffer uniformly removes antibodies from HRP Western blot before reprobing with another set of antibodies.
Promega ECL Western Blotting Substrate
The ECL Western Blotting Substrate is a highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) conjugates on immunoblots.
ProteinSimple Anti-Rabbit Detection Module, 1ea
Use with a Wes or Peggy Sue/Sally Sue Separation Module. Includes: Luminol-S, Peroxide, Antibody Diluent 2, Anti-Rabbit Secondary Antibody, Streptavidin-HRP.
ProteinSimple Anti-Mouse Detection Module, 1ea
Use with a Wes or Peggy Sue/Sally Sue a Separation Module. Includes: Luminol-S, Peroxide, Antibody Diluent 2, Anti-Mouse Secondary Antibody, Streptavidin-HRP.
LI COR BIOTECH LLC CellTag™ 700 Stain for In-Cell Western™ Assays (2 x 10 nmol)
CellTag 700 Stain is a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications. The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers. CellTag 700 Stain is detected in the 700 nm channel of Odyssey CLx, Classic, and Sa Imaging Systems. CellTag 700 Stain is applied to the cells during incubation with an IRDye 800CW secondary antibody, and enables accurate measurement of target protein levels with much higher throughput than Western blotting.
LabScientific NONFAT DRY MILK standard for blotting. Molecular Biology Grade. Western blot. Biotin-Free. 2KG
Standard for all blotting: Western Blot Grade - Tested - Molecular Biology Grade Biotin-Free. 2KG
ProteinSimple RePlex Module for Jess
This RePlex Module provides all the reagents needed to remove primary and secondary antibodies in a RePlex assay with two immunoassays or immunoassay with Total Protein assay in a single run. Please note this module can only be used with Jess.
LI COR BIOTECH LLC NewBlot™ IR Stripping Buffers for NIR Western Blots (100 mL)
NewBlot IR is a robust formulation optimized for near-infrared (NIR) Western blots that works with both nitrocellulose and PVDF membranes. This stripping buffer is a good place to start if you are new to stripping and reprobing NIR Western blots. NewBlot IR works well for removal of all IRDye secondary antibodies, and may be suitable for removal of other dye-conjugated secondary antibodies. NewBlot IR does not require hazardous shipping, unlike many other stripping buffers. Note: NewBlot IR Stripping Buffer is not recommended for loading amounts over 30 µg.
LI COR BIOTECH LLC Revert™ 700 Total Protein Stain Kits for Western Blot Normalization (250 mL kit)
Make Western blot normalization more accurate and reliable with Revert 700 Total Protein Stain, a membrane-based (post-transfer) normalization strategy that stains all protein in your sample. Revert 700 Total Protein Stain works with your existing Western blot workflow and does not require special gels or equipment. The 250 mL Kit is enough for 50 mini blots and contains 1 x 250 mL Revert 700 Total Protein Stain, 1 x 500 mL Revert 700 Wash Solution, and 1 x 500 mL Revert Destaining Solution
ProteinSimple Total Protein Detection Module for Jess, Wes, Peggy Sue or Sally Sue
Use with a Jess/Wes or Sally Sue/Peggy Sue Separation Module. Includes: Luminol-S, Peroxide, Antibody Diluent 2, Total Protein Reconstitution Agent 2, Total Protein Streptavidin-HRP.