General Western Blot Reagents
Azure Biosystems HRP Stripping Buffer
Stripping buffer uniformly removes antibodies from HRP Western blot before reprobing with another set of antibodies.
Azure Biosystems AzureSpectra Labeling Kit, for Western Blot
Kit for covalently labeling antibodies for Western blot with a fluorescent dye. Contains labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns. Each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody
Promega ECL Western Blotting Substrate
The ECL Western Blotting Substrate is a highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) conjugates on immunoblots.
LICOR CellTag™ 700 Stain for In-Cell Western™ Assays (2 x 10 nmol)
CellTag 700 Stain is a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications. The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers. CellTag 700 Stain is detected in the 700 nm channel of Odyssey CLx, Classic, and Sa Imaging Systems. CellTag 700 Stain is applied to the cells during incubation with an IRDye 800CW secondary antibody, and enables accurate measurement of target protein levels with much higher throughput than Western blotting.
ProteinSimple Anti-Rabbit Detection Module, 1ea
Use with a Wes or Peggy Sue/Sally Sue Separation Module. Includes: Luminol-S, Peroxide, Antibody Diluent 2, Anti-Rabbit Secondary Antibody, Streptavidin-HRP.
LICOR Revert™ 700 Total Protein Stain Kits for Western Blot Normalization (250 mL kit)
Make Western blot normalization more accurate and reliable with Revert 700 Total Protein Stain, a membrane-based (post-transfer) normalization strategy that stains all protein in your sample. Revert 700 Total Protein Stain works with your existing Western blot workflow and does not require special gels or equipment. The 250 mL Kit is enough for 50 mini blots and contains 1 x 250 mL Revert 700 Total Protein Stain, 1 x 500 mL Revert 700 Wash Solution, and 1 x 500 mL Revert Destaining Solution
ProteinSimple EZ Standard Pack 1, (8) Single Use Biotinylated Ladder and Single Use FL Standard
8 pk, EZ Standard Pack 1
LICOR Revert™ 700 Total Protein Stain for Western Blot Normalization (250 mL)
Make Western blot normalization more accurate and reliable with Revert 700 Total Protein Stain, a membrane-based (post-transfer) normalization strategy that stains all protein in your sample. Revert 700 Total Protein Stain works with your existing Western blot workflow and does not require special gels or equipment. Revert 700 Total Protein Stain is a robust and replicable solution for Western blot normalization. The stain can be detected in the 700 nm channel of Odyssey Imaging Systems (or competitor systems) for quantification and normalization analyses. The protocol is quick and can be completed within 15 minutes. The 100 mL size is sufficient for 20 mini Western blots (5 mL/blot), and the 250 mL size is sufficient for 50 mini Western blots.
ProteinSimple RePlex Module for Jess
This RePlex Module provides all the reagents needed to remove primary and secondary antibodies in a RePlex assay with two immunoassays or immunoassay with Total Protein assay in a single run. Please note this module can only be used with Jess.
LICOR Revert™ 700 Total Protein Stain Kits for Western Blot Normalization (100 mL kit)
Make Western blot normalization more accurate and reliable with Revert 700 Total Protein Stain, a membrane-based (post-transfer) normalization strategy that stains all protein in your sample. Revert 700 Total Protein Stain works with your existing Western blot workflow and does not require special gels or equipment. The 100 mL Kit is enough for 20 mini blots and contains 1 x 100 mL Revert 700 Total Protein Stain, 1 x 200 mL Revert 700 Wash Solution, and 1 x 200 mL Revert Destaining Solution
LICOR Revert™ 520 Total Protein Stain Kits for Western Blot Normalization (500 mL kit)
Make Western blot normalization more accurate and reliable with Revert 520 Total Protein Stain, a membrane-based (post-transfer) normalization strategy that stains all protein in your sample. Revert 520 Total Protein Stain works with your existing Western blot workflow and does not require special gels or equipment. The 500 mL Kit is enough for 50 mini blots and contains 1 x 500 mL Revert 520 Total Protein Stain, 1 x 1 L Revert 520 Wash Solution, and 1 x 1 L Revert Destaining Solution
ProteinSimple Anti-Mouse Detection Module, 1ea
Use with a Wes or Peggy Sue/Sally Sue a Separation Module. Includes: Luminol-S, Peroxide, Antibody Diluent 2, Anti-Mouse Secondary Antibody, Streptavidin-HRP.
ProteinSimple Total Protein Detection Module for Jess, Wes, Peggy Sue or Sally Sue
Use with a Jess/Wes or Sally Sue/Peggy Sue Separation Module. Includes: Luminol-S, Peroxide, Antibody Diluent 2, Total Protein Reconstitution Agent 2, Total Protein Streptavidin-HRP.