General Western Blot Reagents
Azure Biosystems HRP Stripping Buffer
Stripping buffer uniformly removes antibodies from HRP Western blot before reprobing with another set of antibodies.
Azure Biosystems AzureSpectra Labeling Kit, for Western Blot
Kit for covalently labeling antibodies for Western blot with a fluorescent dye. Contains labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns. Each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody
Promega ECL Western Blotting Substrate
The ECL Western Blotting Substrate is a highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) conjugates on immunoblots.
Bioworld Nitrocellulose Stripping Buffer (10X), 500 mL
Product DescriptionNitrocellulose stripping buffer (10X) is used for hybridization of probes to nucleic acids. It is ideal for removal of secondary antibody after detection of highly abundant targets. It is also used for nucleic acid and protein related blotting.This buffer contains 0.5X SSC and 100 mM EDTA at pH 8.0. To strip the membrane, dilute buffer to 1X in water. Warm solution to 50-70°C and perform 2 to 3 changes (15 minutes each) of boiling stripping buffer.
Bioworld Blot Stripping Buffer II (4X), 500 mL
Product DescriptionBlot stripping buffer II (4x) contains enough liquid concentrate to make 4L of 1X solution. Product is 0.2M glycine and has a pH value of 2.5.To use this product, place the membrane into stripping buffer for 30-45 minutes with gentle agitation. Buffer should be heated to approximately 50°C for incubation. Wash membrane 3 times with TBST or PBST before reprobing.Product is ultra pure grade.
Bioworld Re-Probe Solution western Blots, 125 mL
Re-Probe Solution, Western BlotsRe-Probe is supplied as 5X. The 125ml kit sufficient for 25 blots. Not recommended for chromogenic substrates.
Bioworld Nylon Membrane Stripping Buffer (10X), 500 mL
Product DescriptionNylon Membrane Stripping Buffer (10X) is a liquid used for the hybridization of probes to nucleic acids. Buffer contains Tris HCL at pH 8.0 (1mM), EDTA (100mM), and Denhardt’s solution (1X). To use this product, dilute at 10-fold with sterile deionized water and strip the membrane by 2 hours of incubation at 75°C.